dithiothreitol

🔬 Dithiothreitol (DTT) — Overview

Chemical Identity

• Name: 1,4-Dithiothreitol (DTT)

• Synonyms: Cleland’s Reagent

• Molecular Formula: C₄H₁₀O₂S₂

• CAS Number: 3483-12-3

• Molecular Weight: 154.25 g/mol

• Appearance: White crystalline powder

• Purity: ≥ 99%

• UV Absorbance: < 0.1 at 280 nm for a 1% solution

• Storage: Store at –20 °C

• Solubility: Highly soluble in water, ethanol, chloroform, ether, and ethyl acetate

• Source: Astor Scientific

• 🧪 Applications in Biochemistry & Molecular Biology

  Dithiothreitol (DTT) is a potent reducing agent widely utilized in various biochemical and molecular biology applications:

  • Reduction of Disulfide Bonds: DTT effectively reduces disulfide bonds in proteins and peptides, maintaining them in their reduced state. This property is crucial for protein denaturation and analysis. 

  • Protein Stabilization: By preserving thiol groups in their reduced form, DTT stabilizes enzymes and other proteins, preventing unwanted oxidation. Electrophoresis: DTT is commonly used in SDS-PAGE sample buffers to break disulfide linkages, ensuring proteins are fully denatured for accurate molecular weight determination.

  • Cleavage of Cross-Linkers: It can reduce disulfide bridges in cross-linkers like N,N′-bis(acryloyl)cystamine, aiding in the disruption of polyacrylamide gel matrices.

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  ⚗️ Chemical Properties

  • Redox Potential: DTT has a redox potential of –0.33 V at pH 7, indicating its strong reducing capability. 

  • Molecular Structure: Consists of two thiol (–SH) groups attached to a four-carbon backbone, classifying it as a dithiol and a diol. 

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  🛒 Product Information

  Astor Scientific offers DTT in various quantities:

  • 1g: $36.00

  • 5g: $59.00

  • 10g: $88.00

  • 50g: $371.00 

  For more details and to purchase, visit:
  👉 DTT, 1,4-Dithiothreitol – Astor Scientific